蛋白质组学及其相关技术在运动人体科学中的应用

Application of proteomics and relevant technologies in human exercise sciences

目的:对蛋白组学及蛋白芯片技术发展现状进行综述,为该技术在运动医学中的应用提供参考资料。资料来源:应用计算机检索PubMed2003-01/2006-12期间相关蛋白组学及蛋白芯片技术方面的文章,检索词“exercise AND protein chip,protein microarray”,并限定文章语言种类为English。同时计算机检索万方数据库2003-01/2006-12期间相关蛋白组学及蛋白芯片技术方面的文章,检索词“蛋白质,运动锻炼,运动医学”,并限定文章语言种类为中文。资料选择:对资料进行初审,并查看每篇文献后的引文。纳入标准:文章所述内容应与蛋白质组学及蛋白质芯片技术的研究相关。排除标准:重复研究或Meta分析类文章。资料提炼:共收集到312篇相关文献,32篇文献符合纳入标准,排除的280篇文献为内容陈旧或重复。资料综合:蛋白组学研究已成为基因组学研究后生命科学发展的大方向之一。它研究的主要内容包括:蛋白质分离与鉴定、蛋白质功能的确定、蛋白质翻译后修饰及相互作用、各种疾病或疲劳标志物的筛选与疾病诊断、生物信息学及药物开发等方面。文章在对蛋白质组学的发展及其相关技术在运动人体科学中的应用现状进行综述的基础上,对运动人体科学未来的发展方向进行了展望。由于蛋白质组学的建立以及蛋白质芯片技术的逐步完善,对运动人体科学的研究及其发展将起到很好的促进作用。结论:未来将从分子水平上阐明运动与人体适应的分子生物学机制,研究热点将集中于从运动营养蛋白质组学、反兴奋剂的蛋白质芯片技术、运动员机能评定的蛋白质芯片研究等方面。

OBJECTIVE： To review the development of proteomics and protein chip technique, and provide the references for the application of this technique in exercise science. DATA SOURCES： An online search of PubMed from January 2003 to December 2006 were undertaken to identify the articles of proteomics and protein chip technique, with the searching words Of ＂exercise, protein chip, protein microarray＂, and language was limited to English. Simultaneously Wanfang Database was retneved for related Chinese articles from January 2003 to December 2006, and the searching words were ＂proteomics, protein chip, exercise science＂： STUDY SELECTION： All the datum were checked firstly and the quotation of each paper was examined. Inclusive criteria： the articles related to proteomics and protein chip technique. Exclusive cnteria： the repeated study or the Meta analysis. DATA EXTRACTION： Totally 312 papers were collected, and 32 of them were in accordance with the criteria, while other 280 were deleted due to the outdated content or repetition. DATA SYNTHESIS： Proteomics, secondary to genomics, is crucial for the development of life sciences. Proteomics includes the isolation and identification of protein, definition of protein function, post-translational modification and interaction of protein, the screening of the disease or fatigue marker and disease diagnosis, bioinformatics and drug development. Based on the research progresses and applications of proteomics and protein chip in exercise sciences, this paper analyzes the developing trend of human exercise sciences. The establishment of proteomics and development of protein chip techniques will improve the advancement of human exercise sciences. CONCLUSION： The body＇s adaptation to sports can be further investigated in molecular biological level. Current studies are emphasized on sports nutritional proteomics, anti-doping applications of protein chip and the biochip evaluation of athletes＇ body function.