成纤维细胞生长因子受体3在牙源性肿瘤中的表达(英文)

Expression of fibroblast growth factor receptor 3 in odontogenic tumor

背景:成纤维细胞生长因子受体3与恶性肿瘤的关系已被证实,但是否在具有特殊生物学特性的牙源性肿瘤中扮演角色国内外文献报道较少。目的:观察成纤维细胞生长因子受体3在牙源性肿瘤中的表达。设计:随机对照观察。单位:湖州师范学院医学院口腔医学系,浙江大学医学院附属口腔医院。材料:实验于2003-01/2004-12在浙江大学口腔医学院完成。所有标本均来自浙江大学医学院附属口腔医院病理科1999～2003期间牙源性肿瘤病例,包括造釉细胞瘤29例,角化囊肿20例,始基囊肿36例,以5例正常牙囊或残余牙板上皮作为对照。所有标本均经石蜡包埋,切成3～5μm厚的切片,置于涂有多聚赖氨酸的载玻片上,烘干备用。方法:采用免疫组织化学方法,检测成纤维细胞生长因子受体3在正常牙囊或残余牙板上皮和牙源性造釉细胞瘤、角化囊肿及始基囊肿中的表达。主要观察指标:成纤维细胞生长因子受体3在造釉细胞瘤、角化囊肿、始基囊肿中的表达。结果:成纤维细胞生长因子受体3在造釉细胞瘤、角化囊肿及始基囊肿中呈阳性表达,表达率分别为59%、45%、8%,三者表达差异有显著性意义;在正常牙囊或残余牙板上皮中呈阴性表达。成纤维细胞生长因子受体3阳性细胞集中在肿瘤的细胞成熟区。结论:成纤维细胞生长因子受体3可能与造釉细胞瘤、角化囊肿的发病机制及终末分化机制有关。

BACKGROUND：The relationship of fibroblast growth factor receptor 3 （FBGFR3） and malignant tumor has been confirmed. However, domestic and foreign studies on whether FBGFR-3 plays a role in odontogenic tumor with special biological characteristic are few.OBJECTIVE： To observe the expression of FBGFR-3 in odontogenic tumor.DESIGN： A randomized controlled observation.SETTING： Department of Stomatology, Medical College, Medical College of Huzhou Teachers College; Stomatology Hospital Affiliated to Zhejiang University.MATERIALS：This experiment was carried out in the Stomatology Hospital, Medical College Affiliated to Zhejiang University between January 2003 and December 2004.All the samples were from the odontogenic tumor patients admitted to Department of Pathology, Stomatology Hospital Affiliated to Medical College of Zhejiang University between year 1999 and year 2000, including 29 patients with ameloblastoma, 20 keratocyst and 36 primordial cyst. Normal dental crypt or residual dental lamina epithelium tissue served as control. All the samples were embedded by paraffin, sliced into 3 to 5 μm-thickness sections, placed on polylysine-spread slide, and dried for later use.METHODS： The expressions of FBGFR-3 in the normal dental crypt or residual dental lamina epithelium tissue,odontogenic ameloblastoma, keratocyst and primordial cyst were detected by immunohistochemical method.MAIN OUTCOME MEASURES： Expressions of FBGFR-3 in ameloblastoma, keratocyst and primordial cyst.RESULTS： The expression of FBGFR-3 in the ameloblastoma, keratocyst and primordial cyst was positive, presenting 59%, 45% and 8%, respectively, and there were significant differences in expression of FBGFR-3 among these three;FGGFR-3 was not expressed in the normal dental crypt and residual dental lamina epithelium tissue. FBGFR-3-positive cells gathered in the maturation region of tumor cells.CONCLUSION： FBGFR-3 may be related to the pathogenesis of ameloblastoma and keratocyst, and terminal differentiation mechanism.