不同负荷跑台训练大鼠红细胞免疫功能及血浆β-内啡肽的变化

Red blood cell immunity and plasma beta-endorphin level in rats under different intensity exercises

目的:观察不同负荷运动对大鼠红细胞免疫功能和β-内啡肽含量的影响,并分析两者的关系。方法:实验于2006-05在盐城师范学院运动生理实验室完成。①实验分组:选用3月龄雄性健康SD大鼠50只。随机分为安静对照组10只,常规饲养,不加干预;中等强度运动组20只,进行中等强度跑台训练:第1周速度为22m/min,时间25min;第2周速度为24m/min,时间30min;第3～6周速度为26m/min,时间35～40min;大强度间歇运动组20只,进行大强度间歇跑台训练。第1周速度为32m/min,时间25min;第2周速度为34m/min,时间30min;第3～6周速度为36m/min,时间35～40min。每次大强度跑6min,间歇4min。2个运动组均每周训练5d,每天训练1次。②实验评估:后2组运动后即刻与运动后24h按郭峰建立的红细胞免疫花环试验方法检测大鼠红细胞C3b受体花环率、红细胞免疫复合物花环率。按试剂盒说明测定血浆β-内啡肽水平。结果:完成训练并纳入分析大鼠:安静对照组8只,中等强度运动组14只,大强度间歇运动组14只,共36只。①血浆β-内啡肽水平比较:中等强度运动即刻、大强度间歇运动即刻与大强度运动24h组血浆β-内啡肽含量均明显高于安静对照组(P<0.01),其中大强度间歇运动即刻组血浆β-内啡肽含量最高。中等强度运动即刻与24h组血浆β-内啡肽含量均明显低于大强度间歇运动即刻与24h(P<0.01)。②红细胞C3b受体花环率、红细胞免疫复合物花环率的变化:大强度间歇训练即刻红细胞-C3b受体花环率明显低于安静对照组(P<0.05),中等强度运动后即刻和运动后24h与大强度间歇训练组相近(P>0.05)。中等强度与大强度间歇训练即刻组红细胞免疫复合物花环率均明显高于安静对照组(P<0.05)。③相关性:血浆β-内啡肽含量与红细胞C3b受体花环率之间呈明显正相关(r=0.9114,P=0.011),红细胞-C3bRR与红细胞免疫复合物花环率之间呈明显负相关(r=-0.8534,P=0.031)。结论:不同负荷运动后大鼠血浆β-内啡肽含量升高,红细胞免疫黏附功能下降,运动后血浆β-内啡肽含量的增加有利于红细胞免疫功能的恢复与提高。

AIM： To observe the effects of different intensity exercise training on beta-endorphin and red cell immunity in rats and analyze the relationship between them, METHODS： The experiment was conducted in the Laboratory of Sports Physiology of Yancheng Teachers College in May 2006, ①Fifty healthy male SD rats aged 3 months were randomly divided into three groups. The rats in control group （n =10） only received conventional breeding. The rats in medium intensity exercise group （n =20） received medium intensity treadmill training at the speed of 22 m/rain for 25 minutes in the 1St week, 24 m/rain for 30 minutes in the 2^nd week and 26 m/rain for 35-40 minutes in the 3^rd-6^th weeks. The rats in high intensity intermittence exercise group （n =20） received high intensity intermittence treadmill training at the speed of 32 m/min for 25 minutes in the 1^th week, 34 m/rain for 30 minutes in the 2^nd week and 36 m/min for 35-40 minutes in the 3^rd-6^th weeks, High intensity training was for 6 minutes once with an interval of 4 minutes, The rats in the two training groups were trained for 5 days every week and once a day, ②Rosette rate of red blood cell C3b （RBC-C3b） receptor and rosette rate of red blood cell immunocomplexs （RBC-IC） were determined by Red Blood Cell Immune Rosette Assay designed by Guo immediately and 24 hours after training in the two training groups, Plasma beta-endorphin levels were measured according to the instruction of kit. RESULTS： Totally 36 SD rats including 8 rats from control group, 14 rats from medium intensity exercise group and 14 rats from high intensity intermittence exercise group were involved in the result analysis, ①Plasma beta-endorphin level was obviously higher in the medium intensity exercise group immediately after training and high intensity intermittence exercise group immediately and 24 hours after training than the control group （P 〈 0.01）, and it was the highest in the high intensity intermittence exercise group immediately after training, The plasma beta-endorphin level was clearly lower in the medium intensity exercise group than the high intensity intermittence exercise group immediately and 24 hours after training （P 〈 0,01）, ②Rosette rate of RBC-C3b receptor and rosette rate of RBC-IC： Rosette rate of RBC-C3b receptor was distinctly lower in the high intensity intermittence exercise group immediately after training than the control group （P 〈 0.05）, and the rosette rate of RBC-C3b receptor was similar between the medium intensity exercise group and high intensity intermittence exercise group immediately and 24 hours after training （P〉0.05）. Rosette rate of RBC-IC was remarkably higher in the high intensity intermittence exercise group and medium intensity exercise group immediately after training than the control group （P 〈0.05）, ③ Correlation： Plasma beta-endorphin level was positively correlated with rosette rate of RBC-C3b receptor （r=0.911 4,P=0.011 ）, and the rosette rate of RBC-C3b receptor was negatively correlated with the rosette rate of RBC-IC （r =-0,853 4,P =0.031 ）. CONCLUSION： Plasma beta-endorphin level is elevated and red blood cell immune adherence function is decreased in different intensive exercises, Increased plasma beta-endorphin level can promote the red cell immunity.