摘要
目的观察重组人促红细胞生成素(r-HuEPO)对大鼠颅脑损伤后伤侧脑组织核因子-κB(NF-κB)和Survivin表达的影响,探讨其脑保护机制。方法选取78只健康雄性Wistar大鼠,随机分为假手术组(6只)、颅脑损伤对照组(36只)及r-HuEPO治疗组(36只)。采用自由落体法建立大鼠颅脑损伤模型,应用EpicsXL流式细胞仪检测细胞凋亡情况,并应用免疫组织化学SP法检测Survivin和NF-κB表达的变化。结果与假手术组相比,颅脑损伤对照组的细胞凋亡率及Survivin、NF-κB的表达水平明显升高(P<0.01);与颅脑损伤对照组相比,r-HuEPO治疗组除在伤后6h和7d外,其它各时相点的细胞凋亡率均明显降低(P<0.05),且在伤后1、2、3、5d的Survivin表达及伤后各时间点的NF-κB表达均较明显升高(P<0.05)。结论r-HuEPO可促进NF-κB及抗凋亡因子Survivin的表达而减少颅脑损伤后的细胞凋亡,发挥脑保护作用。
Objective To explore the effect of recombinant human erythropoietin （r-HuEpo） on the expression of survivin after traumatic brain injury （TBI） in rats and the mechanisms of neuro-protection by r-HuEPO. Methods Seventy-eight adult Wistar rats were randomly divided into sham operation group （n=6）, TBI group （n=36） and r-HuEPO group （n=36）. The experimental TBI model was established by Feeney＇s method. The injured cerebral cortexes tissues were obtained 1 day after sham operation in sham operation group and 6 hours and 1, 2, 3, 5 and 7 days after the TBI in TBI and r-HuEPO groups for determining apoptosis of cells by Epics XL Flow Cytometer and the expressions of surviving and NF-κB proteins by immunochemical method. Results Compared to the sham operation, group, the number of apoptotic cells and survivin, NF-κB immunopositive cells were significantly increased in the injuried brain 3, 5 and 7 days after TBI （P〈0.01）. R-HuEPO significantly increased the expression of survivin and NF-KB （P〈0.05）, and decreased significantly the apoptotic rates of cells compared to TBI group（P〈0.05）. Conclusion The expression of NF-κB increased by r-HuEPO might be one of the mechanisms in regulating survivin level, inhibiting neuronal apoptosis in the injury cortex.
出处
《中国临床神经外科杂志》
2007年第6期350-353,共4页
Chinese Journal of Clinical Neurosurgery
基金
全军十一五医学基金资助(06G032)
