摘要
在pH为3．0～4．0的范围内，Cu^2＋会氧化I-并定量析出单质碘，I2与4，6-二溴荧光素反应，使其荧光发生猝灭．据此可建立一种荧光光谱间接测定微量Cu^2＋的方法．初步讨论了I2对二溴荧光素的荧光猝灭机理，认为主要是静态猝灭的作用．该体系的荧光发射波长为λem=531nm．在铜含量12～170μg／mL范围内呈现良好的线性关系，回归方程为△IF=0．3473C＋1．5466（C：μg／mL），相关系数r=0．9989，检出限为5．3μg／mL．将该方法用于水样中铜离子的测定，得到较好的结果．
A fluorimetric method has been developed for microdetermination of copper ion in water with 4,6-dibromofluorescein. It＇s based on the fundamentals that Cu^2＋ can oxidize I^- and generate element iodine at pH = 3.0 -4.0, and I2 can reacte with dibromofluorescein and make the fluorescence being quenched. The mechanic of reaction is discussed and wavelength of the solution system＇s fluorescence-emission is λem=531 nm. The calibration graph is linear over the range 12-170μg/mL with the equation △IF = 0. 347 3 C＋1. 546 6（C： μg/mL）, r =0. 998 9. and the detection limit is 5.3μg/mL. The method has been applied successfully to the determination of copper ion in the water with a satisfactory results.
出处
《泉州师范学院学报》
2007年第2期71-74,共4页
Journal of Quanzhou Normal University
关键词
4
6-二溴荧光素
荧光猝灭法
碘
铜离子
4,6 - dibromofluorescein
fluorimetric method
iodine
copper ion
