酪氨酸为底物的过氧化物酶催化荧光反应

Peroxidase Catalytic Fluorescence Reaction with Tyrosine as Substrate

G.G.Guilbault等人曾对过氧化物酶催化H_2O_2氧化高香草酸(HVA)的反应进行过较详尽的研究。利用这个反应可以对有关的氧化还原酶、辅酶、有机底物、无机离子和微量H_2O_2进行分析检测。但是,以高香草酸为底物,价格昂贵,作为通用检测方法不够经济。Guilbault认为酪氨酸有类似的反应,且价格便宜,但未进行详细研究。我们在此基础上。

Fluorescence behaviours of L-Tyr-H2O2-HRP system were investigated. The results indicate that tyrosine is not equal to homovanillic acid for the structure of the substrate, but tyrosine and ho-movanillic acid have the same linear ranges and sensitivities in the enzymatic analysis, and tyrosine can replace homovanillic acid. Therefore, it is possible to make good use of that reaction. The method has been used to determine glucose and tyrosine in human sera and the results are obtained satisfactory.