猪圆环病毒2型陕西株的分离鉴定

Isolation and identification of PCV2 in Shaanxi

根据GenBank中猪圆环病毒1型(PCV1)和2型(PCV2)的序列,设计2对PCR引物,对疑似PCV2感染猪的血液、肺脏和淋巴结等病料进行套式PCR扩增检测,并对PCR检测为PCV2阳性的病料进行PCV2分离与鉴定。结果显示,PCV2在PK-15细胞上生长良好,但增殖较慢且不产生细胞病变;在第12代细胞培养物中用间接免疫荧光试验(IFA)和PCR扩增反应均能检测到PCV2,且特异性很强。表明试验分离的病毒为PCV2。

Two pairs of primers are designed according to the sequences of PCV1 and PCV2 in the GenBank to detect PCV2 in the blood,lungs and lymph nodes of borderline cases by nest PCR. Then the PCV2 positive samples are used to separate the virus. Results show that PCV2 are well cultivated on the PK-15 cell,but there is no CPE. PCV2 can be found on the twelfth generation of the cell by IFA and PCR,and the specificity is very high,which indicates the virus separated in this experiment is PCV2.