玉米幼芽提取物对小鼠皮肤成纤维细胞Ⅰ、Ⅲ型前胶原mRNA表达的影响

Effect of extracts of Maize plummule on expression of type Ⅰ procoll-agen and type Ⅲ procollagen in cultured mouse dermal fibroblasts

分离培养小鼠皮肤成纤维细胞,用2种富含腺嘌呤类衍生物的玉米幼芽提取物(20 mg/L)提前保护细胞后,体外构建H2O2急性氧化损伤细胞模型,应用RT-PCR对小鼠皮肤成纤维细胞Ⅰ、Ⅲ型前胶原mRNA的表达进行半定量检测。结果表明,损伤对照组(Ⅱ组)小鼠皮肤成纤维细胞Ⅰ、Ⅲ型前胶原mRNA的表达量分别为空白对照组(I组)的24%(P<0.01)和20%(P<0.01),而药物保护组(Ⅲ组、Ⅳ组)小鼠皮肤成纤维细胞Ⅰ型前胶原mRNA的表达量均高于损伤对照组,分别是损伤对照组的3.5倍(P<0.01)和1.8倍,Ⅲ型前胶原mRNA的表达量也均高于损伤对照组,分别是损伤对照组的4.6倍(P<0.01)和3倍(P<0.01)。说明在H2O2致小鼠皮肤成纤维细胞急性氧化损伤过程中,这2种玉米幼芽提取物能够保护Ⅰ、Ⅲ型前胶原mRNA的表达,且0501提取物对小鼠皮肤成纤维细胞Ⅰ、Ⅲ型前胶原表达的保护作用优于0502提取物。

The mouse dermal fibroblasts had been separated from mouse skin and oxidative damage cell mode was cultured by H2O2 in vitro, after two kinds of Maize plurnrnule extracts were used in test groups before damaging. The expression of type Ⅰ procollagen and type Ⅲ procollagen （mRNA level） were examined by semi-quantitative RT-PCR. The results showed that the type Ⅰ procollagen and type Ⅲ procollagen mRNA expression in the H2O2 damaged group were only 24% （P〈0.01） and 20% （P〈0.01） of that in the control group. The type Ⅰ procollagen mRNA expression in two test groups by using two kinds of Maize plurnrnule extracts is 3.5 higher （P 〈 0.01） and 1.8 higher than that of the H2O2 damaged group respectively;the type Ⅲ procollagen mRNA expression in two test groups is 4.6 higher （P 〈 0.01） and 3 higher （P 〈 0.01） than that of the H2O2 damaged group,respectively. So,two kinds of Maize Plurnrnule extracts effectively protected the mRNA expression of type Ⅰ procollagen and type Ⅲ procollagen from the short-time oxidative damage by H2O2 in mouse dermal fibroblasts culture. The 0501 extracts had a better protection for the mRNA expression of type Ⅰ procollagen and type Ⅲ procollagen in mouse dermal fibroblast than that of the 0502 extracts.