四倍体刺槐扦插生根过程中氧化酶活性的变化

Studied on the oxidation enzymes activities in the periods of making roots in the green branch of Robinia pseudoacacia

四倍体刺槐(Robinia pseudoacacia)属难生根树种,为了解其生根过程中氧化酶活性的变化规律,用300,400和500 mg/L的吲哚丁酸(IBA)处理四倍体刺槐嫩枝插条,以清水浸泡为对照,测定扦插生根过程中体内吲哚乙酸氧化酶(IAAO)、多酚氧化酶(PPO)和过氧化物酶(POD)活性的变化。结果表明,各IBA处理和对照插穗IAAO和POD活性变化趋势相似,在四倍体刺槐生根过程中呈升-降-升-降的变化规律,而PPO酶则基本呈降-升-降-升的变化规律;不同处理间3种酶活性的平均值和各阶段酶活性差异均显著。说明在四倍体刺槐扦插生根过程中,不定根的发生和发展与3种酶活性有密切联系,不同浓度IBA处理对各酶活性影响显著,其中以500 mg/L IBA处理对四倍体刺槐扦插生根的促进效果更明显。

The Robinia pseudoacacia is a kind of trees with difficult growing roots. The green branch of Robinia pseudoacacia footings are treated with the indoleacetic butyric acid （IBA）,then the activities of indoleacetic acid oxidase（IAAO）,polyphenol oxidase（PPO） and peroxidase（POD） are tested regularly. During the different periods of taking roots,the three enzymes activities vary differently; the indoleacetic acid oxidase（IAAO） and the peroxidase（POD） vary alike; the polyphenol oxidase（PPO） activities are contrary to the indoleacetic acid oxidase（IAAO） and peroxidase（POD）. Besides, in the different periods of taking roots,the three enzymes activities show significant difference,and the different process of the enzymes activities also have remarkable difference,and the 500 mg/L of indoleacetic butyric acid （IBA） has the best marked improvement on the making roots of the Robinia pseudoacacia.