金标免疫层析试条检测样品中黄曲霉毒素B_1的误差分析

Study on error analysis during detecting aflatoxin B_1 in food by using Gold Labeled Immucochromatographic Strip

为了扩大金标免疫层析试条(GICA)检测食品和饲料中黄曲霉毒素B1的应用范围,研究了样品基质对试剂条信号强度的影响,探讨了GICA测定饲料和各种发酵类食品样品时,提取液中NaCl、金属离子(Fe3+,Cu2+,Pb2+,As3+)和脂肪含量对检测结果的影响,并研究了消除影响的方法。结果表明,3种阴性样品(大米、玉米和小麦粉)均存在不同程度的非特异性干扰,大米的影响最小,其次为玉米和小麦粉,大米的最佳稀释倍数为10倍,玉米和小麦粉为30倍;随NaCl质量浓度增加,检测结果有偏向假阳性的趋势;4种金属离子对检测结果的影响程度依次为Fe3+>Cu2+>Pb2+>As3+;随着样品中油脂含量的增高,用GICA检测结果偏阳性的程度加剧。对粗提取液用氯仿法萃取后挥干再溶解,可将盐离子和重金属离子对测定的影响控制在允许范围之内;用石油醚脱脂,可使油脂对测定结果的影响降到最低。

The key factors, including NaCl, metal ions （Fe^3＋ , Cu^2＋ , Pb^2＋ , As^3＋ ）, oil content and so on,were studied by detecting all kinds of ferment foods and feed, to amplify the application region of the Gold Labeled Immucochromatographic Strip （GICA） in detecting aflatoxin B1 in foods. The results showed that all the factors had to some degree serious influence on the detecting results. The three negative samples （rice, corn and wheat flour） had no specific disturbance influence on detection. The sequence was corn, flour〉rice. The best dilution multiples of rice were 10,while corn and wheat flour were 30;With the increasing of concentration of NaCl,the muscaline ratio of decting results are increasing;according to the influence on the result, the sequence among those factors is Fe^3＋ 〉Cu^2＋ 〉Pb^2＋ 〉As^3＋. The experiment showed that with the increasing of lipin in the samples,the muscaline ratio increased,too. Fe^3＋ ,Cu^2＋ , Pb^2＋ and As^3＋ extracted by chloroform, volatilizated and then dissolved could ensure the influence under the limited line. Removed the oil by petroleum,the influence caused by oil could be decreased in the lowest line.