人肺腺癌多药抗药细胞系LC-3/CDDP细胞周期及DNA含量变化

Cell Cycle and DNA Content in a Cisplatin-resistant Subline of Human Lung Cancer Cell Line

应用流式细胞仪测定人肺腺癌多药抗药细胞系LC－3／CDDP之细胞周期、DNA指数，用3H-胸腺嘧啶核苷掺入法测定DNA合成动态，结果显示，LC－3／CDDP细胞系G2＋M峰较亲代细胞明显增高，G期细胞比例降低，S期、G2＋M期比例升高，DNA指数增加，DNA合成速率较亲代细胞显著增快。提示人肺腺癌多药抗药细胞系LC－3／CDDP对DNA损伤的修复功能增强。

In order to investigate the mechanisms of multi - drug rdsistance in LC- 3/CDDP, a cisplatin - re-sistant subline of human lung cancer cell line, cell cycle, DNA index and mpential DNA synthesis werestudied.The results showde that the percentage in phases and G2+ M of the resistant cell line increasedmore sibnificantly than that of its cell line. DNA index increased remarkadly in LC-CDDP,which was corrdsponded with the sequentialoborvation of DNA synthesis. We conlude that the increasedDNA repair in LC - 3/CDDP might be one of the mechanisms of multi- drug resistance.