摘要
石刁柏胚性愈伤组织继代过程中,添加浓度为10 mg·L^(-1)的外源腐胺能有效地保持愈伤组织的胚胎发生能力,并减少愈伤组织的褐化,但不能提高愈伤组织的体细胞胚的诱导率。腐胺处理过的胚性愈伤组织的内源腐胺含量明显提高。这可能是外源腐胺保持细胞胚性、降低褐化程度的原因。
Asparagus officinalis embryogenic calli, subcultured on media with 10 mg·L^-1 exogenous putrescine, exhibited longer embryogenic competence and less browning. However, induction of somatic embryos was not promoted by application of exogenous putrescine. Compared with the non-putrescine treatment （control）, embryogenic calli pretreated with putrescine exhibited a high level of the endogenous putrescine. It is suggested that exogenous putrescine could prompt calli embryogenic competence and reduce calli browning due to the accumulation of endogenous putrescine.
出处
《植物生理学通讯》
CSCD
北大核心
2007年第3期461-464,共4页
Plant Physiology Communications
基金
教育部留学生基金(教外司1999363)
国家自然科学基金(30540019)。
关键词
石刁柏
愈伤组织胚胎发生能力
褐化
内外源腐胺
Asparagus officinalis
callus embryogenic competence
browning
exogenous and endogenous putrescine
