特发性肺间质纤维化患者血清细胞因子的检测及其意义

Detection and significance of serum cytokines levels in patients with idiopathic pulmonary fibrosis

目的探讨血清TGF-β1、IL-13水平在特发性肺间质纤维化(IPF)发生发展中的意义。方法采用双抗体夹心ELISA法检测36例IPF患者治疗前与治疗3个月后血清中TGF-β1、IL-13水平。结果IPF患者血清TGF-β1、IL-13水平明显高于正常对照组(P<0.05)。血清TGF-β1、IL-13水平治疗有效组较治疗前降低(P<0.05),治疗无效组较治疗前升高(P<0.05)。无效恶化组血清TGF-β1、IL-13水平明显高于有效组(P<0.05)。结论IPF患者体内高水平的TGF-β1、IL-13与IPF的发生发展密切相关,有可能作为检测病情,判断疗效的客观指标以及IPF的辅助诊断指标。

Objective To investigate the significance of expression of transforming LigTowth factor β1 （TGF-β1） and interleukin- 13 in serum of patients with idiopathic pulmonary fibrosis（IPF）. Methods The serum levels of TGF-β1 and IL-13 in 36 patients with IPF were detected by ELISA before and after 3 - month treatment. Results The serum levels of TGF-β1 and IL-13 in patients with IPF were significantly higher than those of control group. In the effective group, the serum TGF-β1 and IL-13 levels were decreased, as compared with. In the non-effectivegToup, the serum TGF-β1 and IL-13 levels were increased. The serum TGF-β1 and IL-13 levels in effective group were decreased, as compared with those in non - effective group. Conclusion The increase of TGF-β1 and IL-13 levels is related in the development and progression of IPF. TheLiserum TGF-β1 and IL-13 levels might represent some useful parameters in monitoring the changes of disease state and evaluating therapeutic effect on IPF.