甘氨脱氧胆酸对人肝癌细胞增殖抑制与诱导凋亡的实验研究

Experimental study on growth inhibition and apoptosis induced by glycodeoxycholic acid in human hepatocellular carcinoma cells

目的探讨甘氨脱氧胆酸（GDCA）对人肝癌细胞SMMC7721增殖的影响以及细胞凋亡作用。方法采用96孔细胞培养板体外培养人肝癌细胞株SMMC7721，在24、48、96h后加入GDCA，并进行四甲基偶氮唑蓝（MTT）实验，计算肝癌细胞的生长抑制率；采用流式细胞仪检测细胞周期和细胞凋亡的影响。结果GDCA对肝癌细胞SMMC7721有增殖抑制作用，GDCA作用细胞72h后，200、400、800／μmo／L GDCA抑制率分别为18．18％、29．94％和88．54％；细胞周期分析显示，G1期细胞比例与对照组相比明显升高，GDCA200、400／μmol／L处理组P〈0．05，S期有不同程度的下降，GDCA400／μmol／L处理组P〈0．01。Annexin V-FITC检测结果显示GDCA 200、400μmol／L处理组48和72h后细胞凋亡比例比对照组明显增加，差异具有统计学意义。结论GDCA对人肝癌细胞有增殖抑制作用，并能诱导SMMC7721细胞凋亡。

To explore effects of glycodeoxycholic acid （GDCA） on cell proliferation and apoptosis induction of human hepatocellular carcinoma SMMC7721 cells. Methods SMMC7721 cells were cultured in 96-well cell culture plate and different concentrations of GDCA were added into the wells respectively. The proliferation ability was observed with MTT assay at 24, 48, 72 h after culture, and then the inhibitor rate was calculated. The apoptotic rate of SMMC7721 and cell cycle were analyzed by flow cytometry after GDCA treatment. Results GDCA inhibited the proliferation of human hepatocellular carcinoma SMMC7721 cells. The inhibition ratio of cell proliferation in different group of GDCA 200,400, and 800 /μmo/L was 18.18%, 29.94% and 88.54% respectively 72 h after GDCA treatment. Cell cycle analysis showed the numbers of G1 stage cells increased in different GDCA treatmen＇t groups than that of the control group. There were statistical difference in GDCA 200 and 400/μmol/L group as compared with that of the control group （P〈0.05）. The numbers of S stage cells decreased to some extent. In GDCA 400 μmol/L group, the difference was significant. The apoptotic ratios in 200 and 400 μmol/L GDCA group increased obviously as compared with that of the control group after 48h and 72h by Annexin V-FITC analysis （P〈0.05）. Conclusion GDCA can inhibit the cell proliferation of human hepatocellular carcinoma SMMC7721 cells and induce their apoptosis.