阴道分泌物中支原体培养及耐药性分析

Culture and analysis of drug resistance of mycoplasma, from vaginal secretions

目的了解本地区非淋菌性尿道炎（NGU）患者阴道分泌物支原体感染情况及其对抗菌药物的耐药性，指导临床合理用药。方法用法国生物梅里埃公司的IST2试剂盒对280例NGU女性患者的阴道分泌物进行支原体培养及药敏试验。结果在280例NGU患者中，支原体阳性161例，阳性率为57．5％，其中解脲支原体（Uu）为主要病原体，占阳性率的87．0％，人型支原体（Mh）占阳性率的2．5％，Uu和Mh混合感染占阳性率的10．5％。药敏结果显示，抗菌活性较好的抗生素是原始霉素、强力霉素、交沙霉素，其次是四环素；耐药率最高的是环丙沙星、氧氟沙星，其次是红霉素和阿奇霉素。结论对于Uu和Mh感染，临床医生应根据药敏试验结果合理选用抗生素，目前本地区妇科支原体感染患者可选择强力霉素和交沙霉素作为首选抗生素。

To investigate infection rate and drug resistance of mycoplasma from vaginal secretions of patients with non-gonococcal urethritis in our area and to guide the rational drug use in clinic. Methods IST2 kit （Biomerieux Company） was used to culture mycoplasma in 280 cases of vaginal secretions of patients with non-gonococcal urethritis and to observe its drug sensitivity. Results Among the 280 NGU patients, 161 cases were with mycoplasma culture positive （57. 5%）. The major pathogen was Ureaplasma urealyticum （Uu）, accounting for 87.0% in all positive samples; Mycoplasma hominis （Mh） was 2.5%; Uu and Mh was 10.5%. The result of drug sensitivity test showed that antibiotics with high antimicrobial activity were Pristinamycin, Doxycycline, Josamycin, and the next was Tetracycline. Ciprofloxacin and Ofloxacin had higher resistance rate, and the next was Erythromycin and Azitromycin. Conclusion Antibiotics should be used rationally based on drugsensitivity test results during Uu and Mh infection treatment. At present, Doxycycline and Josamycin may be used as the first antibiotics to treat mycoplasma infection in our area.