云南拉祜族群体15个STR基因座遗传多态性研究

Polymorphism Investigation on 15 STR Loci of Yunnan Lahu Population

目的采用PCR-STR及基因分型技术,探讨云南地区拉祜族群体D3S1358、TH01等15个常染色体基因座的遗传多态性,建立拉祜族群体的遗传学基础数据,为群体遗传学和法医学应用提供依据.方法采集101名云南临沧地区拉祜族无关个体的静脉血,经典有机溶剂法提取DNA,应用PCR技术,复合扩增D3S1358、TH01等15个常染色体基因座的短串连重复序列,扩增产物用遗传分析仪毛细管电泳荧光检测,调查云南地区拉祜族群体15个STR基因座等位基因频率,进行遗传多态性分析.结果15个STR基因座在云南拉祜族群体中具有遗传多态性,基因型分布符合Hardy-Weinberg平衡定律(P>0.05).其累积匹配概率(PM)为4.36×10-16,累积非父排除率(CPE)为99.9994%,累积个人识别能力(TDP)为99.9999999999999%.结论云南拉祜族群体15个常染色体STR基因座有较高的非父排除率和个体识别能力,可为法医学亲子鉴定和个体识别及遗传学研究提供依据.

Objective To study the autosomal loci polymorphism, such as D3S1358 and TH01, in Yunnan Lahu population to construct genetic basic data of Lahu population and provide foundation for population study and forensic cases. Methods Venous blood was collected from 101 unrelated Lahu individuals in Lincang, Yunnan province. DNA was extracted by classical organic solvent extraction. PCR technique was applied to amplify the sequence of autosomal loci. The products were measured by electrical fluoroscopy. The genet frequency of 15 STR loci were investigated and genetic polymorphism was analyzed. Results Genetic polymorphism of 15 STR loci was detected and the distribution of genotype conformed with Hardy - Weiberg balance law （ P 〉 0. 05 ） ： cumulative matching probability （PM） was 4. 36 × 10^-16, cumulative non - parental eliminant rate （CPE） was 99. 999 4% , and cumulative individual Discriminative power （CDP） was 99. 999 999 999 999 9%. Conclusion The 15 STR loci used in this study are highly polymorphic in Lahu population and can be applied to population study and forensic practice.