胸苷磷酸化酶在乳腺癌组织中的表达及其与预后的关系分析

Primary analysis of the expression of TP in breast cancer and its relationship with prognosis

目的研究胸苷磷酸化酶(thymidine phosphorylase,TP)在乳腺癌组织中的表达及其和预后的关系。方法采用免疫组织化学S-P法,检测72例乳腺癌病例及15例良性乳腺肿瘤组织和15例正常乳腺组织中TP的表达差异,并分析其与预后的关系。结果在72例乳腺癌组织中,有59例(81.9%)TP表达阳性,与在正常乳腺组织和在乳腺良性肿瘤组织中的表达(分别为46.7%和53.3%)差异显著。多因素Cox模型分析显示腋窝淋巴结转移、C-erb-B2表达和治疗方式为影响预后的独立因素。在行含5-FU方案化疗的乳腺癌亚群中进行生存分析,TP阳性组与TP阴性组的五年总生存率差异有显著性。结论TP在乳腺癌中高表达,明显高于乳腺良性肿瘤和正常乳腺组织表达量。在以5-FU为基础的乳腺癌联合化疗中TP阳性组预后较TP阴性组预后为佳。

[Objective] To investigate the expression of thymidine phosphorylase in breast cancer and its relationship with prognosis. [Methods] We examined the expression of TP in 72 samples of breast cancer using immunohistochemistry S-P method. 15 samples of fibroma tissues and 15 samples of normal human breast tissues were assessed as controls. Factors that influence the long-term survival of breast cancer patients were analyzed. [Results] TP was positive in 59/72 （81.9%） samples, which was significantly different from that in normal breast tissues （46.9%） and in benign breast tumors （53.3%）. The multiple-factor Cox＇s model indicated that metastasis of axillery lymph nodes, and expression of C-erb-B2 and therapy pattern could be independent factors of prognosis. Carrying out survival analysis in the subgroup of breast cancer patients who received chemotherapy based on 5-FU, in which Log-rank test showed a significant difference in 5-year overall survival time between TP positive group and TP negative group. [Conclusions] TP overexpressed in breast cancer, which was significantly higher than that in normal breast tissues. In the combined chemotherapy based on 5-FU, the prognosis was better in TP positive patients than in TP negative patients. The Cox＇s model indicated that TP might not be independent factors of prognosis.