阿魏酸对人脐静脉血管内皮细胞ECV304的增殖作用

Proliferation effect of Ferulic acid on endothelial cells ECV304 of human umbilical vein

目的:观察阿魏酸对于培养人脐静脉内皮细胞ECV304增殖的影响,为治疗性血管新生的靶基因选择提供依据。方法:取对数生长期的内皮细胞,分为阿魏酸低、中、高浓度组,同步化于G0期,ELISA法检测BrdU的掺入;RT-PCR法检测血管内皮细胞生长因子(VEGF)mRNA的表达情况。结果:与对照组相比,不同浓度阿魏酸均可显著提高BrdU的掺入量,上调VEGFmRNA的表达,且在102ng/ml～104ng/ml之间呈一定的量效关系。结论:阿魏酸能显著促进体外培养的ECV304细胞的增殖,可能具有血管新生的作用。

Objective:To observe the proliferation effect of ferulic acid on the endothelial cells ECV304 of human umbilical vein, so as to find a candidate way for therapeutic angiogenesis. Method:We incubated cells with FA (102ng/ml, 103ng/ml, 104ng/ml) for 24 h after synchronizing the ECV304 at G0, BrdU-ELISA assay was performed to test the DNA synthesis. The expression of VEGF mRNA by RT-PCR was also observed. Result:Compared with control, different concentration of ferulic acid can improve the BrdU incorporation and enhance the expression of VEGF mRNA with a dose-dependent manner raging from 102ng/ml to 104ng/ml. Conclusion:Ferulic acid can significantly improve the proliferation of ECV304 and play an important role in angiogenesis.