2-甲氧基雌二醇对大鼠神经元及C6胶质瘤细胞增殖与凋亡的影响

Effect of 2-methoxyestradiol on proliferation and apoptosis of neurons and C6 glioma cells in rats

目的:探讨2-甲氧基雌二醇(2-methoxyestradiol,2-ME)对大鼠神经元及C6胶质瘤细胞增殖及凋亡的影响。方法:将神经元及C6胶质瘤细胞分为对照组和实验组,实验组按2-ME浓度不同分为1、5、10、15μmol/L 4个亚组。2-ME作用于大鼠神经元及C6胶质瘤细胞不同时间后,采用MTT法检测细胞活性,流式细胞仪检测细胞凋亡和细胞周期,并在光镜及电镜下观察其形态学变化。结果:C6胶质瘤细胞经2-ME作用后细胞活性受到抑制,呈现时间变量依赖性,各亚组与对照组差异具有统计学意义(P<0.05)。2-ME可诱导C6胶质瘤细胞凋亡,各亚组与对照组差异具有统计学意义(P<0.05);可使C6胶质瘤细胞G1及S期细胞减少,G2期细胞增多,一定范围内呈现剂量依赖性,各亚组与对照组差异具有统计学意义(P<0.05)。神经元经药物作后,各亚组与对照组细胞活性差异无统计学意义。结论:2-ME可抑制C6胶质瘤细胞生长,诱导C6胶质瘤细胞凋亡,而对正常神经元无影响。

Objective： To investigate the effect of 2-methoxyestradiol on growth inhibition and apoptosis of C6 glioma cells and neurons in vitro. Methods： C6 glioma cells and neurons were divided into the control group and four 2-methoxyestradiol treatment groups. C6 glioma cells and neurons were treated by different doses of 2-methoxyestradiol for different times and observed by microscopy and electromicroscopy. The viability was determined by MTT assay and the cell-cycle and apoptosis were determined by flow cytometry. Results： 2-methoxyestradiol caused a dose and time-dependent inhibition on the C6 glioma cells viability（ P 〈 0.05）. The population of C6 glioma cells in the G1 and S phase was decreased while in the G2 phase it was increased by 2-methoxyestradiol in a dose and time-dependent manner（ P 〈 0.05）; However, 2-methoxyestradiol had no effect on neurons. Conclusion： 2-methoxyesradiol can inhibit proliferation and induce apoptosis of C6 glioma cells, but has no effect on neurons.