雷帕霉素对培养的人Tenon′s囊成纤维细胞的作用

Role of rapamycin on cultured human Tenon′s capsule fibroblasts

目的:观察雷帕霉素(rapamycin)对培养的人Tenon′s囊成纤维细胞的增殖及细胞周期的作用。方法:将培养的人Tenon′s囊成纤维细胞用含不同雷帕霉素浓度的培养液培养24 h后,用MTT法测定细胞的增殖情况,用流式细胞仪测定细胞周期的变化情况。结果:用含10、20、40、80和160 ng/ml雷帕霉素的培养液培养细胞时,细胞增殖抑制率分别为9.32%,22.55%,41.42%,42.25%,43.57%。细胞周期分析显示,雷帕霉素(40 ng/ml)作用后,G0/G1期细胞较对照组增多(41.76%vs 44.19%,P<0.05),S期细胞较对照组减少(45.42%vs 40.18%,P<0.05)。结论:雷帕霉素具有抑制人Tenon′s囊成纤维细胞增殖的效果,且随浓度增加抑制作用增强,并将细胞抑制在G1期的限制点内。

Objective： To explore the effect of rapamycin on the proliferation and differentiation of human Tenon＇s capsule fibroblasts in cell cycles. Methods： The cultured fibroblasts were treated in DMEM medium containing various concentrations of rapamyein for 24 hours. The growth of fibroblasts was measured by a methylthiazlyl tetrazolium （MTT） assay. The flow cytometry method was used to evaluate the cell cycles. Results： Proliferation of fibroblasts were suppressed by rapamycin from a dose of 10 ng/ml to 160 ng/ml（ P 〈 0.05）. The cell growth inhibition rate treated with mpamycin concentrations of 10 ng/ml, 20 ng/ml, 40 ng/ml, 80 ng/ml, and 160 ng/ml were 9.32%, 22.55%, 41.42%, 42.25%, and 43.57% respectively. The cell population was significantly increased at the G0/C1 phase from 41.76% to 44.19% and decreased at the S phase from 45.42% to 40.18% after the rapamycin treatment. Conclusion： Rapamycin is a potential inhibitor that blocks the proliferation of human Tenon＇s capsule fibroblasts at the C1 restriction point.