棕包梨的RAPD分析

RAPD analysis of Zhongbao pear

通过建立梨RAPD反应的优化体系对棕包梨和其他梨品种进行聚类分析,探讨了它们之间的亲缘关系.结果表明:西洋梨(Pyrus communis L.)与东方梨[(砂梨(P.pyrifdia Burn)和白梨(P.bretschneideri Rehd)]品种之间亲缘关系较远;而砂梨与白梨品种之间亲缘关系较近;棕包梨与砂梨、日本梨品种之间的亲缘关系较远;而棕包梨与蜜雪梨间的亲缘关系很近,而蜜雪梨为我国台湾横山梨和日本水晶梨的杂交品种.表明我国台湾的横山梨和福建的棕包梨可能是同一品种类型.

An optimal random amplified polymorphic DNA（RAPD） reaction system was established to analyse the relationships among Zhongbao pear and other pear cultivars, The cluster analysis results were as follows： Pyrus communis L. could be distinctly distinguished from P. pyrifdia Burn and P, bretschneideri Rehd; P. pyrifdia Burn. and P. bretschneideri Rehd. were not clustered into different groups respectively; Zhonghao pear could be distinctly distinguished from cultivars of P. pyrifdia Burn. and P. bretschneideri Rehd; Zhongbao pear and Mixue pear were clustered into a group, while Mixue pear was the crossing variety of Hengshen pear and Shuijing of Japan. It was suggested that Zhongbao pear and Hengshan pear of Taiwan were likely same cultivar.