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PCR结合DGGE技术快速鉴定橘小实蝇及其近缘种 被引量:2

Rapid identification of 6 species of fruit fly(Diptera:Tephritidae) by PCR-DGGE
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摘要 首次采用PCR结合变性梯度凝胶电泳(DGGE)技术对橘小实蝇及其近缘种的线粒体COⅡ和16S rDNA基因序列进行分析.结果表明,通过DGGE可以区分实蝇属不同种类的COⅡ和16S rDNA基因序列. Bactrocera dorsalis (Hendel) and the other tive Bactrocera species ( B. latifrons, 1t. tau, 11. lateriataenia, 11. scuteUata, B. cucurbitae) were identified by PCR-DGGE method based on the gene analysis of mtDNA CO Ⅱ and 16S rDNA. The result showed PCR-DGGE was a rapid and specific technique for pest species identification in plant quarantine.
出处 《福建农林大学学报(自然科学版)》 CSCD 北大核心 2007年第3期274-278,共5页 Journal of Fujian Agriculture and Forestry University:Natural Science Edition
关键词 橘小实蝇 检疫 PCR-DGGE 种类鉴定 Bactrocera dorsalis (Hendel) quarantine PCR-DGGE(denaturing gradient gel electrophoresis) species identification
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