SRB法检测大鼠原发肝癌细胞、骨髓间充质干细胞和HepG2细胞的抗肿瘤药物敏感差异

Differences in chemosensitivity to anticancer drugs among hepatocarcinoma cells,bone marrow mesenchymal stem cells and HepG2 cells by SRB assay in rats

目的:应用SRB法探讨不同药物对骨髓间充质干细胞(MSCs)、肝癌细胞(HC)和HepG2细胞的药敏差异.方法:雌性清洁级SD大鼠20只,应用二乙基亚硝胺饲喂法建立大鼠原发性肝癌模型,选取诱癌成功大鼠8只分别原代培养HC与MSCs,同时培养HepG2细胞系细胞,SRB法进行药物敏感实验,观察药物敏感性的差异.结果:对阿霉素、顺铂、洛拉曲克的敏感度从高到低依次为HepG2细胞,HC,MSCs.而丝裂霉素药敏显示,3种细胞药物敏感度从高到低依次为HepG2,MSCs,HC.结论:HC药敏实验与HepG2细胞和MSCs存在一定异同.

AIM： To study the differences in chemosensitivity to 4 anticancer drugs among hepatocarcinoma cells, MSCs （marrow mesenchymal stem cells ） and HepG2 cells by SRB assay. METHODS： The primary liver carcinoma in rats was induced by diethylnitrosamine. Tumor cells and MSCs from 8 hepatocarcinoma rats were separated . The inhibition rates of hepatocarcinoma cells, MSCs and HepG2 cells were measured and analyzed by SRB assay. RFSULTS： The chemosensitivity of hepatocarcinoma cells and HepG2 cells to adriamycin, cisplatin and nolatrexed dihydrochloride were higher than that of MSCs, while the chemosensitivity of HepG2 cells and MSCs to MCC was higher than that of hepatocarcinoma cells. CONCLUSION： The MSCs have a distinct chemoresistance mechanism like the tumor stem cells.