丹酚酸B对兔离体心脏缺氧再复氧损伤的保护作用

Protective effect of salvianolic acid B against anoxia-reoxygenation injury in isolated rabbit hearts

目的观察丹酚酸B对兔离体心脏缺氧-复氧损伤的保护作用。方法离体兔心脏Langendorff灌流,通氮气饱和的灌流液60min后再恢复含氧灌流液灌流60min,造成缺氧-复氧损伤;自动生化分析仪测定冠脉流出液中肌酸激酶(CK)和乳酸脱氢酶(LDH)活性;光学显微镜观察心肌组织结构改变。结果在缺氧的同时给予丹酚酸B0.3,1和3mg.L-l灌流60min,可降低心率,增加冠脉流量,降低冠脉流出液中CK和LDH的水平,心肌组织形态学损伤明显减轻。结论丹酚酸B对兔离体心脏缺氧-复氧损伤有明显的保护作用。

AIM To investigate the protective effect of salvianolic acid B against the myocardial injury in isolated rabbit hearts caused by anoxia-reoxygenation （A-R） to confirm the known cardioprotective effect in vivo. METHODS A-R model hearts perfused on Langendorff apparatus were subjected to hypoxic perfusion for 60 min, and followed with 60 min of normoxic perfusion. Salvianolic acid B 0.3, 1.0 and 3.0 mg· L^-1 were perfused in the whole course of hypoxia for 60 min, respectively. The activities of creatine kinase （CK） and lactate dehydrogenase （LDH） in coronary flow were measured, and histopathological changes in hearts were observed under light microscope. RESULTS Salvianolic acid B perfusion decreased heart rate and increased coronary flow in hearts injured by A-R. CK and LDH activities in coronary flow were much lower, and morphological lesions were improved as compared to A-R model hearts. CONCLUSION Salvianolic acid B offers myocardial protective effect against A-R injury.