摘要
通过对番红花及其混淆品的rDNA ITS区序列进行PCR扩增、测序,并运用Clustal X、Mega 3.0等软件进行序列分析.结果表明番红花rDNA ITS区序列全长650 bp,GC百分比为60.3%,与其混淆品的rDNA ITS区序列存在着显著差异.另外,还设计出了鉴别番红花的位点特异性PCR引物,无需测序即可对番红花及其混淆品进行准确的分子鉴别.
The PCR products of rDNA ITS regions of Crocus sativus L. and its adulterants were sequenced and analyzed using Clustal X,Mega 3.0 software. The results showed that the full length of rDNA ITS regions of C. sativus was 650 bp, and the percentage of GC contents was 60. 3% , which displayed the remarkable distinction between rDNA ITS regions of C. sativus and its adulterants. In addition, a pair of allele-specific PCR （AS- PCR） primers were designed to authenticate C. sativus and its adulterants accurately without sequencing.
出处
《南京师大学报（自然科学版）》
CAS
CSCD
北大核心
2007年第2期89-92,共4页
Journal of Nanjing Normal University(Natural Science Edition)
基金
江苏省普通高校高新技术产业发展项目基金(JH02-124)资助项目
关键词
番红花
rDNA
ITS区
混淆品
位点特异性PCR
分子鉴别
Crocus sativus L. , rDNA ITS region, adulterant, allele-specific PCR, molecular authentication
