摘要
以沸石为填料用吸附法构建了氧化亚铁硫杆菌固定化细胞生物反应器,考查了空气流量、液体流量对固定化效果的影响。在温度相同,培养基的pH=1.6,Fe2+浓度为8g/L左右的条件下,固定化细胞在Fe2+氧化率达95.36%时的氧化速率高达1.10g/L.h,其Fe2+的氧化速率将近是游离细胞的18倍。固定化细胞生物反应器的动力学研究表明,氧化亚铁硫杆菌固定化细胞氧化Fe2+反应符合一级反应,其反应速率常数k为0.37h-1。
The immobilization cell bioreactor of Thiobacillus ferrooxidans was built with zeolite as support particles. The effects of air flow and liquid flow on immobilization were researched. When the conversion ratio of ferrous ions of immobilization cell was 95.36% , the oxidation velocity of ferrous ions of immobilization cell was 1.10g/L· h, and was about eighteen times that of free cells under the conditions of the same temperature, pH 1.6 and concentration of ferrous ions about 8g/L. The study of kinetics of immobilization cell bioreactor indicated that the reaction of ferrous ions oxidized by immobilization cell was in conformity with the first - order reaction, and the reaction rate constant k was 0. 37h^-1.
出处
《云南冶金》
2007年第3期24-27,77,共5页
Yunnan Metallurgy
