混合血清经量值传递后用于酶活性测定的定标

Calibration of enzymatic activity determination using home-made mixed serum transmitted by quality

目的探讨混合血清经酶校准品量值传递后,用于标定血清酶活性定量测定的因子数的可行性。方法用Trucal U复合校准血清和经量值传递的混合血清分别定标血清丙氨酸氨基转移酶(ALT),门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、γ-谷氨酰转肽酶(GGT)、活性测定的因子数以及理论K值计算法,分别测定20份新鲜血清样品中四种酶的活性。结果1、TruCal复合标准血清标定后的测定结果与理论K值计算法测定结果比较,ALP、GGT的相对偏倚分别为-25.21%和15.67%,作回归分析和配对t检验,(t=15.644和3.846,P<0.001)。与经量值传递的混合血清标定后的测定结果比较,ALP、GGT的相对偏倚分别为2.31%和0.98%。2、混合血清标定后,测定高、中、低三个水平的混合血清及TruCal U复合校准血清,CV%、相对偏倚均小于5%,但低值样品的ALT、AST的CV%为9.08和7.68。结论经量值传递的混合血清标定血清酶活性定量测定的因子数,可以提高血清酶测定的因子数,可以提高血清酶活性测定的准确度,使血清酶活性定量测定的结果有可溯源性。

Objective To investigate the feasibility of using home-made mixed serum transmitted by quality to calibrate calibration factor in enzymatic activity determination. Mechods Factors were calibrated in enzymatic activity measurement of ALT,AST,ALP,GGT with TruCal U calibrator serum and home-made mixed serum separately,and then the enzymatic activity of ALT,AST,GGT in twenty fresh serum was determined with K-value calculation. Resuits 1. By comparing the results calibrated by TruCal O calibrator serum with the results calculated with K-value calculation,the relative variability of ALP and GGT was -25. 21% and 15.76 %. Then results of regression analysis and t-test were t= 15. 644 and 3. 846,P〈0. 001. While by comparing the results calibrated by home-made mixed serum with the results calibrated by TruCal U calibrator serum,the relative variability of ALP and GGT was 2.31% and 0. 98%. 2. Both CV% and relative variability were less than 5% in mixed serum of high,media,low grade and TruCal U calibrator serum,but CV% of ALT,AST in low grade was 9. 08 and 7. 68. Conclusion The accuracy and measurement traceability can be improved when home-made mixed serum is used to calibrate enzymatic activity measurement.