参麦对肝缺血再灌注一氧化氮、内皮素-1影响的研究

Influence study of Shenmai on nitric oxide and endothelin-1 following hepatic ischemia reperfusion injury

目的观察一氧化氮(NO)、内皮素-1(ET-1)在肝缺血再灌注损伤过程中的变化及参麦对其的影响。方法随机分为3组:对照组(C组,n=30)、缺血再灌注组(IR组,n=30)和参麦治疗组(She组,n=30)。分别测定缺血前、缺血第20分钟及再灌注第45分钟时肝组织NO,ET-1含量,用电镜观察经She保护后的肝组织形态学改变。结果肝缺血再灌注第45分钟时,与C组、She组比较,IR组血浆及组织NO含量均明显较低(P<0.01,P<0.05);IR组血浆及组织ET-1含量明显较高(P<0.01,P<0.05);与IR组比较,She组肝组织超微结构明显改善。结论参麦可通过调节NO和ET-1水平对肝缺血再灌注损伤发挥很好的保护作用。

Objective It is to observe the changes of nitric oxide （NO） and endothelin- 1 （ET- 1 ） in the course of hepatic ischemia reperfusion injury and the influence of Shenmai on them. Methods The patients were randomly divided into group C （n ：30）, group IR （n =30） and group She （n =30）. The contents of NO and ET- 1 in hepatic tissue were respectively determined before ischemia, after ischemia for twenty min, after reperfusion for 45 min. Morphological changes of hepatic tissue after Shenmai protection were observed with electron microscope. Results The contents of NO in plasma and tissue in group IR after hepatic ischemia reperfusion injury for 45 min were obviously lower than those in group C and group She （P〈 0.01 and P 〈 0,05）, and at the same time the contents of ET- 1 in plasma and tissue in group IR were obviously higher than those in group C and group She （P 〈 0.01 and P 〈 0,05）. Compared with group IR, the ultrastructural of hepatic tissue in group She was obviously improved. Conclusion Shenmai can educe better protective effect on hepatic ischemia reperfusion injury through adjusting the levels of NO and ET- 1.