宫颈鳞癌中P120ctn及相关蛋白的表达及临床意义

Expression of P120ctn and Related Proteins in Squamous Cervical Carcinoma and Its Significance

目的：研究P120连环蛋白（P120ctn）、β连环蛋白（β—cat）、E钙粘蛋白（E—cad）及Ki67蛋白在宫颈鳞癌中的表达及临床意义，分析P120ctn异常表达与B—cat、E—cad及Ki67蛋白异常表达的相互联系。方法：应用免疫组化SP法检测P120ctn、β—cat、E—cad及Ki67蛋白在26例正常宫颈组织和52例宫颈鳞癌组织中的表达。结果：①正常宫颈组织中P120ctn、β—cat及E—cad表达在胞膜，胞浆少见；宫颈鳞癌中P120ctn及E—cad胞膜表达降低，出现胞浆表达，异常表达率分别为41／52（78．8％），38／52（73．0％）；β—cat除了胞浆表达以外，还伴随胞核表达，异常表达率为39／52（75％）；P120ctn、β—cat及E—cad异常表达与肿瘤细胞分化程度、淋巴结转移有关（P〈0．05），与年龄、临床分期无关（P〉0．05）；②同一组织标本中P120ctn表达异常，同时伴β-cat及E—cad异常表达；③正常宫颈组织中Ki67表达率为4／26（16％），宫颈鳞癌组织中表达率为40／52（80％）；Ki67异常表达与临床分期有关（P〈0．05），与年龄、肿瘤细胞分化程度及淋巴结转移无关（P〉0．05）；P120ctn异常表达与Ki67蛋白异常表达有相关性。结论：P120cm、β-eat及E—cad在宫颈鳞癌中异常表达，三者异常表达有很好一致性，P120ctn异常表达与Ki67蛋白异常表达有相关性。P120ctn及相关蛋白异常表达可作为宫颈鳞癌诊断的辅助指标，并为判断疾病发展及转移提供客观依据。

Objective： To research the relationship between abnormal expression of P120 - catenin and clinic pathologic significance in squamous cervical carcinoma（SCC） , β - catenin, E - cadherin and Ki67 protein. Methods： Expression of P120 - catenin, 13 - catenin, E - eadherin and Ki67 protein were examined in 26 cases of normal cervical epithelia samples and 52 cases of SCC by immunohistochemical staining （SP method）. Results： ① In the normal cervical epithelia tissue, P120 - catenin, β - catenin and E - cadherin expression showed on the membrane and lowly in the cytoplasm; In the SCC, P120 -catenin and E - cadherin expression were found in cytoplasm, lower in the membrane, abnormal exoression rates were 41/52 （78. 8% ）, 38/52 （73. 0% ） respectcticely; β -catenin expression showed in the cytoplasm and nuclea, abnormal expression rates was 39/52（75% ） ; Abnormal expression of P120 - catenin, β - catenin and E - cadherin were significantly correlated with status of tumor differentiation and lymph node metastasis（P 〈 0.05 ） , no significant association was found between abnormal expression and clinical stage and age（ P 〉 0.05 ）. ②P120 - catenin abnory expressed, as well as β - catenin and E - cadherin in identical organization imen. ③ In normal cervical tissue, expression rates of Ki67 protein was 4/26 （ 16% ） , In SCC , the expression rates was 40/52 （80%） ; Abnormal expression of Ki67 protein was significantly correlated with clinical stage, no significant correlated with tumor differentiation status, lymph node metastasis and age （P 〉 0.05 ） ; There are relationship between abnormal expression of P120 - catenin and Ki67 protein. Conclusions： In SCC, P120 - catenin, β - catenin and E - cadherin showed abnormal expression, the expressions of three proteins above have highly conformance, there are relationship between abnormal expression of P120 -catenin and Ki67 protein, abnormal expression of P120ctn and related proteins may be useful markers for diagnosis of SCC, and provide a objective fact for assessing development and metastasis of scc.