缺血预处理对大鼠脑缺血再灌注损伤保护作用的探讨

Study of the Protective Effects of Ischemic Preconditioning on Cerebral Ischemia - reperfusion Injury in Rats

目的：探讨缺血预处理对大鼠脑缺血再灌注损伤的保护作用及其机制。方法：SD大鼠随机分为三组，分别为假手术组、脑缺血再灌注组、脑缺血预处理组。各组采用Zea—longa等的大脑中动脉线栓法并加以改进，制备缺血预处理及缺血再灌注损伤实验动物模型。比较各组神经功能缺失评分、脑梗死体积、血清NSE含量及脑组织IL-1β和TNF-α含量。结果：脑缺血预处理可使神经功能缺损减轻、脑梗死体积缩小、血清NSE含量降低，脑组织IL-1β和TNF—α含量降低。结论：缺血预处理可诱导脑缺血耐受作用的产生，下调脑组织再灌注损伤时IL—1β和TNF—α的表达，对脑缺血再灌注损伤具有保护作用。

Objective： To investigate the protective effects and the mechanisms of cerebral ischemic preconditioning on cerebral ischemia - reperfusion injury of rats. Methods： SD adult rats were divided into 3 groups randomly： sham group, cerebral ischemia - reperfusion group, brain ischemic preconditioning group. The rat model of focal cerebral ischemia was created by middle cerebral artery occlusion （MCAO） after Zea - longa by making some modification. Then comparing neurological deficits scores and the volume of cerebral infarction. The level of NSE in se- rum and the level of IL - 1β and THF -α in cerebral tissue were also detected. Resuits： Brain ischemic preconditioning can decrease neurological deficits and reduce the volume of infarction. At the same time, the level of NSE in serum can be decreased. The level of IL - 1β and TNF - α in cerebra｜ tissue also decreased. Conelusions： Brain ischemic preconditioning can induce the effect of ischemic tolerance and decrease the expression of IL - 1β and TNF - α. So brain ischemic preconditioning has protective effects on cerebral ischemia - reperfusion injury in rats.