健康高龄者外周血NK细胞活性及肿瘤坏死因子分泌的研究

Study on NK cell activity and TNF secretion of healthy elderly

目的为探讨健康高龄者外周血自然杀伤细胞的活性及经诱导活化后肿瘤坏死因子(tumor necrotic fac-tor,TNF)的分泌。方法采用乳酸脱氢酶释放法,以K562细胞和SGC-7901细胞为靶细胞测定外周血NK细胞的活性;白介素2(IL-2)与脂多糖诱导活化后的TNF的分泌活性测定以L-929细胞作为靶细胞,用MTT法测定其存活率表示。结果与健康中青年组比较,高龄组人群的NK细胞对K562和SGC-7901细胞的杀伤活性均降低,但差异无统计学意义(P>0.05),经IL-2与LPS诱导的外周血淋巴细胞分泌的TNF活性均增高,差别有显著性(P<0.01和P<0.05)。结论高龄组人群的外周血淋巴细胞经诱导分泌的TNF活力增高可能补偿了部份免疫功能的下降。

Objective To study the NK cell activity and tumor necrotic factor（TNF） secretion of peripheral blood of the healthy elderly. Methods The NK cell activity was detected with LDH release of K562 cell and SGC-7901 cells; The release of TNF of L-929 cells induced by IL-2 and LPS were detected with MTT method. Results NK cell activity of NK cell to K562 cell and SGC-7901 cells were lower, but secretion of TNF induced by IL-2or LPS of peripheral blood lymphocyte were higher as compared to that in the adults （P 〈 0.01;P〈0.05） .Conclusion The secretion of TNF of peripheral blood lymphocyte induced by IL-2 or LPS may partly compensate the immune function for the elderly.