摘要
目的:采用反相高效液相色谱法测定替莫普利盐酸盐及其有关物质。方法:以岛津C18柱(5μm,250mm×4.6mm)为分析柱。乙腈:0.05mol/L磷酸二氢钾缓冲液并用磷酸调节pH值至3.0=44:56为流动相,流速1.0mL/min,检测波长为210nm。结果:标准曲线线性范围为150～350μg/mL(r=1.0000)。最低检测限和定量限分别为7.16ng和23.87ng。结论:方法简便、准确、灵敏,可用于莫昔普利的常规分析。
It is develop an RP-HPLC method to determine Moexipril hydrochloride and its related substances. Methods： The Cls column （5μm,250mm ×4.6mm） was used as analytical column.Mobile phase consisted of acetonitrile ：0.05mol/L mixed solution of potassium dihydrogen phosphate buffer solution=44：56, flow rate was 1.0mL/mino The detection wavelength was set at 210nm. Results： The standard curve was linear in the concentration range from 150to 350μg/mL for Moexipril hydrochloride （r=1.0000）. The limit of detection and quantitation for the assay were 7.16 and 23.87ng, respectively. Conclusion： The method is simple, accurate and sensitive, and can be used for the routine analysis of resveratrol.
出处
《浙江化工》
CAS
2007年第6期29-30,共2页
Zhejiang Chemical Industry
