摘要
目的建立高效液相色谱-蒸发光散射检测法同时测定熟地黄饮片中葡萄糖、果糖的含量。方法色谱柱:ZorbaxRX-SIL(4.6×200mm);流动相:乙腈-水(95∶5);流速:1.0mL.min-1;柱温:25℃;ELSD:漂移管温度105℃,载气流速2.5mL.min-1。结果果糖在1.00~11.00μg范围内(r=0.99996),葡萄糖在0.95~10.45μg范围内(r=0.99991)线性良好,平均回收率分别为97.93%、98.65%,RSD分别为2.07%、2.48%(n=5)。结论该方法操作简便,可用来控制熟地黄饮片的质量。
OBJECTIVE To develop an HPLC--ELSD method for determination of fructose and glucose in Radix Rehmanniae Preparata. METHODS Chromatographic column: Zorbax RX- SIL( 4. 6× 200mm) ; Mobile phase: acetonitrile--water (95 : 5) ; flow rate: 1.0mL · min^-1. Column temperature: 25℃ ; ELSD: temperature of drift tube, 105℃ ; gas flow, 2.5mL · min^-1. RESULTS The assay displayed good linearity,the linear range of fructose was 1.00-11.00μg( r =0. 99996)and that of glucose was 0.95-10. 45μg( r =0. 99991). The average recovery( n =5) was 97.93%( RSD =2.07%) and 98.65%( RSD =2.48%). CONCLUSION The method is simple and can be used for controlling the quility of Radix Rehmanniae Preparata
出处
《齐鲁药事》
2007年第6期338-340,共3页
qilu pharmaceutical affairs