红树植物内生真菌Penicillium sp.中两个甾体化合物的研究

Isolation and Structures of Two Steroids from Mangrove Endophyte Penicillium sp.

从红树植物内生真菌Penicillium sp.的发酵液中分离纯化了两个甾体类化合物,通过各种波谱实验(1D-NMR,2D-NMR,ESI-MS)确定为:麦角甾-4,6,8(14),22-四烯-3-酮(1)和麦角甾-5,7,22-三烯-3-醇(2),1对于3α-HSD脱氢酶在250μm浓度下有较弱的活性。

In our continued chemical investigation on the marine fungi, two steroids were isolated from the fermentation broth of marine fungus PeniciUium sp. purified from mangrove Kandelia candel. On the basis of NMR （ ^1H NMR,^13C NMR, COSY, HMQC, HMBC ） and ESI-MS data, they were identified as ergosta- 4,6,8 （ 14 ） ,22-tetraen-3-one （ 1 ） and ergosta-5,7,22-trien-3-ol（2）. Compound 1 possessed weakly effect on 3or-Hydro xysteroid Dehydrogenase （3ct-HSD） inhibitory activity at 250μm.