雪莲水溶性多糖提取工艺的研究

Study on Extraction Process of Water Soluble Polysaccharide From Saussurea involucrata

雪莲多糖是一种水溶性的功能性天然多糖,作者比较了料液比、温度、提取时间等因素对新疆雪莲组织培养物中雪莲多糖提取率的影响。结果表明,雪莲水溶性多糖的最佳提取工艺条件为料液比为1 g∶12 mL,温度85℃,时间2 h.粗多糖最大得率为7.08%。此粗多糖总糖质量分数为15.37%,从糖含量的角度,新疆雪莲组织培养物提取的粗多糖是野生雪莲提取的粗多糖的优良替代物品。

To optimize the quality and quantity of polysaccharides from Saussurea involucrata, a water-soluble polysaccharide was extracted from Saussurea involucrata by method of boiling water and ethanol deposition. Reagent proportion ,reaction temperature and reaction time were selected with substitution degree and yield as index by L9 （3^3） orthogonal design. The result shows that the optimal extraction conditions were 85 ℃, 2 h as well as the ratio 1 - 12 v/v. By Combination with the optinums condition, a high crude polysaccharides（7.08%） was obtained, and the content of polysaccharide was 15.37%.