壳聚糖微球固定化胰蛋白酶的研究

The Immobilization of Trypsin on Chitosan Microspheres

以壳聚糖为载体,将胰蛋白酶固定化,通过优化其制备条件提高活力回收。首先利用反相悬浮交联法制得壳聚糖微球并用于胰蛋白酶的固定,后采用中心复合设计及响应面分析法优化固定化条件,结果表明,戊二醛体积分数为16.2%,分散时间为98 min,给酶量1 mg/g载体时,固定化胰蛋白酶的活力和活力回收率可达到42.02 u/g和73.32%。

In this manuscript, Trypsin was immobilized on chitosan and the preparing conditions was optimized to achieve higher activity recovery. The chitosan microspheres were prepared by the inverse suspension crosslinking technique and applied to the immobilizatiori of trypsin. Then, the central composite design and response surface analysis were adopted to determine the optimal levels. The optimum conditions were as follows： 16.2% solution glutaraldehyde, dispersing for 98 minutes and 1mg of enzyme per g wet carrier and under which the activity and activity recovery of immobilize trypsin could reach 42.02 u/g and 73.32%.