摘要
L-阿拉伯糖异构酶能将D-半乳糖异构成D-塔格糖。通过单因素试验和快速登高法对乳酸菌SK1.002产L-阿拉伯糖异构酶的培养基进行优化,确定发酵优化条件为(组分g/L):麦芽糊精28,酵母膏10,玉米粉浆22,无水乙酸钠10,K3PO40.2,NaCl 0.01,FeSO4.7H2O 0.01,Mg-SO4.7H2O 0.2,MnSO4.2H2O 0.05,L-阿拉伯糖2.5。发酵初始pH 8.4,培养温度37℃,接种体积分数3%,培养时间12 h。在此发酵条件下,酶活达到了7.28 U/mL。
L-arabinose isomerase mediates the isomerization of D-galactose into D-tagatose. In order to increase the production of L-arabinose isomerase, the culture medium of Lactobacillus SK1. 002 was optimized through single-factor and fast uphill tests. The optimum medium as follow（g/L）：maltodextrin 28, yeast extract 10, corn steep liquor 22, CH3 COONa 10, K3PO4 0. 2, NaCl 0.01, FeSO4 · 7H2O 0.01, MgSO4 @ 7H2O 0. 2, MnSO4 · 2H2O 0.05, L-arabinose. 2. 5 The optimum fermentation conditions were initial pH 8.4, cultivate temperature 37 ℃, inoculation volume 3%, fermentation time 12 h. With the optimal nutritional and environmental conditions, the enzyme activity reached 7.28 U/mL.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2007年第3期100-105,共6页
Journal of Food Science and Biotechnology
关键词
乳酸菌
L-阿拉伯糖异构酶
塔格糖
培养基优化
发酵条件
Lactobacillus
L-arabinose isomerase
tagatose
medium optimization
fermentation condition
