转基因大豆及其深加工产品的PCR检测

Detection of transgenetic soybean and soybean products by PCR method

以PCR技术为基础,建立了从大豆及其深加工产品中检测转基因成分的方法。大豆及其深加工产品采用改良的CTAB法进行DNA提取纯化,大豆色拉油DNA则用试剂盒方法进行了提取纯化。对提取的DNA用PCR方法对大豆特异性内源基因lectin进行扩增,设计CaMV35启动子和NOS终止子特异性引物对其是否含有转基因成份进行初步的定性PCR筛选,并用抗除草剂基因CP4EPSPS对阳性结果进行确证。实验结果表明,改良的CTAB法对大豆深加工产品的DNA有很好的提取效果,而试剂盒方法对色拉油的DNA有良好的提取效果;PCR检测转基因的方法快速高效,检测结果与标准相符。

A method that detected the genetically modified organisms （GMO） in soybean and soybean products had been established on the basis of PCR technique. The DNA of soybean and its products was extracted and purified by means of the modified CTAB method. The DNA of soybean salad oil was purified by a commercial eatraction kit. The purified DNA was detected by qualitative PCR using soybean lectin gene which was one of soybean endogenesis reference genes, CaMV35S promoter and NOS terminator specific primers. The positive samples were also tested by the target gene, 5′-enolpyruvylshikemate-3-phosphate synthase gene （CP4EPSPS） which induced herbicide tolerance in modified soybean. The results showed that the CTAB method is suitable for DNA extraction of soybean and its products, but the kit is more effective in DNA extraction of salad oil. The qualitative PCR is a quick and effective way to screen the GMO.