清颜如玉方治疗寻常痤疮实验研究

Experiment study of "Qinyanruyu Decotion" on curing acne vulgaris

对中药复方清颜如玉方进行药效学研究。用肉汤稀释法,37℃培养金黄色葡萄球菌、表皮葡萄球菌48h,37℃培养痤疮丙酸杆菌72h,定量测定清颜如玉方最小抑菌浓度;观察该方对二甲苯致小鼠耳廓肿胀的影响;并建立兔耳试验模型,观察清颜如玉方的抗角化作用。清颜如玉方对金黄色葡萄球菌、表皮葡萄球菌、痤疮丙酸杆菌均有明显的抑菌作用,最小抑菌浓度分别为10.8mg·mL-1、43.2mg·mL-1、43.2mg·mL-1;能减轻二甲苯所致小鼠耳廓肿胀;对兔耳试验痤疮模型的毛囊扩张、毛囊内角化物质增多以及真皮层炎细胞浸润都有明显的减轻作用。中药复方清颜如玉方有明显的抗炎、抑菌、抗毛囊角化作用。

To study the pharmacodynamics of ＂Qinyanruyu Decotion ＂. Propinibacterium acnes were anaerobic cultured at 37 ℃ for 72 hours, staphylococcus aureus and staphylococcus epidermis were cuhrued at 37 ℃ for 48 hours with MIC method.Observed the most tolerance dosage. Observed that effected auricle swelling induced by dimethylbenzene. Established an experimental animal model for acne to observe the anticomedone effect of ＂Qinyanruyu Decotion＂. The minimumin hibitory concentration of ＂Qinyanruyu Decotion 40 were 10.8 mg/mL, 43.2mg/mL and 43.2 mg/mL by quantitative determination.The decotion could significantly eased auricle swelling induced by dimethylbenzene and reduce the follicular keratinous plug and the dermal inflammationl of the experimental animal model of acne. The esperimental results indicated the decotion had obvious antibacterial, anti - inflammatory and anti follicular hyperkeratosis effects.