血管内皮生长因子在人年轻恒牙及成熟恒牙牙髓中的表达

Expression of vascular endothelial growth factor in dental pulp of immature and mature permanent teeth in human

目的:研究人年轻恒牙和成熟恒牙牙髓中血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达及分布特征,探讨VEGF在恒牙牙根发育过程中的作用。方法:将因正畸或阻生拔除的健康牙分为2组,第1组年轻恒牙10例,第2组根尖闭合的成熟恒牙15例。对牙髓标本的石蜡切片进行VEGF的免疫组化染色,通过Image pro-plus5.1图像分析软件,对图像进行定量分析,采用SPSS13.0软件包进行χ2检验、t检验、单因素方差分析及SNK-q检验。结果:VEGF在年轻恒牙牙髓成纤维细胞和成牙本质细胞胞质呈强阳性表达,显著强染于成熟恒牙(P<0.05);年轻恒牙根髓在喇叭状基顶区成纤维细胞VEGF染色强阳性,以此处向冠方及根方,VEGF表达逐渐减弱。结论:VEGF在人年轻恒牙和成熟恒牙牙髓组织中呈不同特征的表达,VEGF参与恒牙牙根的发育和成熟。

PURPOSE： To investigate the expression and distribution of vascular endothelial growth factor （VEGF） in human dental pulp of immature and mature permanent teeth, and the relationship between VEGF and root development of human permanent teeth. METHODS： The normal teeth extracted for orthodontic or impacted reason were divided into two groups： group 1 contained 10 immature permanent teeth and group 2 contained 15 apical closed mature permanent teeth. The pulps were processed with immunohistochemically staining of VEGF. Image pro-plus 5.1 software was used to evaluate the histological sections and the data was analyzed by Chi-square test, Students＇t test, one-way ANOVA and SNK-q test with SPSS 13.0 software package. RESULTS： The expression of VEGF was strongly positive in the cytoplasma of pulp fibroblasts and odontoblasts in immature permanent teeth,significantly higher than in mature permanent teeth （P〈0.05）. The expression of VEGF was demonstrated most strongly positive in fibroblasts in the top area of apical pipe socket of immature root pulp, and gradually weakly positive toward the coronal and apical direction. CONCLUSIONS： The expression of VEGF in dental pulp of immature and mature permanent teeth shows different characteristics. VEGF may play a role in permanent tooth development and maturation.Supported by Natural Science Foundation of Shandong Province（Grant No. Y2006C48）.