玉米抗镰刀菌穗腐病接种方法及抗病资源筛选研究

Inoculation Technique and Screening Maize Germplasm Resistance to Fusarium Ear Rot

对玉米抗穗腐病性鉴定中采用的牙签法与花丝通道注射法进行比较研究表明,牙签法接种果穗的发病程度重于花丝通道注射法接种。牙签法接种病菌于果穗子粒与穗轴之间,有利于病原菌的发育扩展,是一种比较理想的、容易操作的接种方法。采用牙签法接种串珠镰刀菌,对178份玉米自交系和15份杂交种进行抗穗腐病鉴定,筛选出高抗(HR)自交系1份、抗病(R)玉米自交系34份、抗病(R)玉米杂交种12份。

Two methods, toothpick inoculation and silk channel inoculation ,were compared for screening the maize germplasm resistance to Fusarium ear rot. The results indicated that toothpick inoculation caused higher severity of maize ear comparing with that of silk channel inoculation. The toothpick inoculation was a desired and easy to operate technique for screening the resistance, the pathogen in between kernel and cob was favor infection and promoted establishment of the pathogen. A total of 178 maize inbred and 15 hybrids were evaluated for resistance to ear rot by using toothpick inoculation in the field. Among the tested maize inbred and hybrids, one highly resistant and 34 resistant maize inbred, and 12 resistant hybrids were screened for the disease resistance to Fusarium ear rot.