维生素B_6对大鼠早期糖尿病肾病的防治作用

Effects of Vitamin B_6 on Prevention and Treatment of Early Diabetic Nephropathy in Rat Models

目的:探讨维生素B6对糖尿病肾病(DN)大鼠肾功能的保护作用及可能的机制。方法:利用链脲菌素(STZ)腹腔注射法诱导建立DN大鼠模型,将其随机分为模型组(D组)、维生素B6治疗组(B组)、氨基胍治疗组(A组),并与正常对照组(C组)比较,每组10只大鼠;A、B组每天分别用氨基胍、维生素B61g溶于1L蒸馏水中,C、D组仅给予1L蒸馏水,24 h后自由饮用。观察治疗期间大鼠的一般状况、空腹血糖(FBG)、糖化血红蛋白(HbA1c)、血三酰甘油(TG)、血总胆固醇(TC)、血肌酐(SCr)、肾小球滤过率(GFR)、24 h尿白蛋白排泄率(UAER)、肾小球提取物糖基化终末产物(GTE-AGEs)、肾组织病理等改变。结果:(1)造模组大鼠均出现肾脏功能损害。(2)维生素B6对FBG及HbA1c无影响,但能降低DN大鼠的TG、TC、SCr、24h-UAER,增加GFR。(3)维生素B6能降低DN大鼠肾小球AGEs,其作用效果与氨基胍相当。结论:(1)维生素B6无降糖作用,但对肾脏功能有一定的保护作用;(2)维生素B6能降低糖尿病大鼠的高脂血症;(3)维生素B6具有与氨基胍相似的抑制肾脏AGEs在糖尿病大鼠肾脏蓄积的作用,发挥其对糖尿病肾病的防治作用。

Objective：To explore the effect of Vitamin B6 on renal function of streptozotocin （STZ）-induced diabetic nephropathy （DN）rats and its mechanism. Methods： Diabetic nephropathy rats induced by intraperitoneal injection of STZ were randomized into PN model group （group D, n = 10 ）, Vitamin B6 group （group B, n = 10 ）and aminoguanidine group（ group A, n = 10 ）, contrasted to anormal control group （ group C, n = 10 ）. Aminoguqnidine and VitB6 with 1 g dissolved in 1 L distilled water were given to group A and B,while group C and D only had 1 L disstided water. All groups drunk water freely after 24 hours. During and after the treatment, the changes in general condition, fasting blood glucose （FBG） levels, hemoglobin A1 c （HbA1 c ） , blood triglyceride （TG） , total blood cholesterol （TC） , serum creatinine（SCr）, 24-hour urinary microalbumin excretion rate （UAER）, clearance of creatinine glomerular filtration rate （GFR）, glomerular tissue extracts AGEs （GTE-AGEs） and renal tissue morphology were observed in different groups. Results：Diabetes mellitus and renal function impairment occurred in the model groups. Vitamin B6 had no effects on FBG and HbA1c levels, while it decreased TG, TC, SCr and 24 h UAER and increased GFR. Vitamin B6 also shown similar effects of aminoguanidine on GTE-AGE reduction. Conclusion ： Vitamin B6 has no effect in decreasing blood sugar level, but has a definite effect in renoprotection, and decreases hyperlipidemia in diabetic rats. Vitamin B6 showed similar effects with aminoguanidine on parameters measured, which supports a mechanism of involving AGEs inhibition.