4-羟基壬烯醛诱导支气管上皮细胞凋亡

4-hydroxynonenal induces the apoptosis of bronchial epithelial cells

目的探讨4-羟基壬烯醛(4-HNE)对支气管上皮细胞(16-HBE)凋亡的诱导作用及其机制。方法将支气管上皮细胞(16-HBE)分为空白对照组、10μmol/L、30μmol/L、50μmol/L4-HNE作用4h组,观察4-HNE作用4h后的细胞凋亡情况。Western印迹方法检测磷酸化(p-)SAPK-JNK和caspase-3蛋白表达的情况。结果细胞经30μmol/L、50μmol/L4-HNE作用后,姬姆萨染色可见明显细胞凋亡改变。对照组、10μmol/L、30μmol/L、50μmol/L4-HNE组的AnnexinV-PI染色凋亡细胞数分别为1.94±1.03,2.03±1.04,43.36±1.3,65.92±3.45。30μmol/L和50μmol/L4-HNE组与对照组及10μmol/L4-HNE组比较,差异有统计学意义(P<0.01)。各组p-SAPK-JNK蛋白表达差异无统计学意义(P>0.05)。30μmol/L、50μmol/L4-HNE刺激组caspase-3的表达较对照组和10μmol/L4-HNE组差异有统计学意义(P<0.01)。结论30、50μmol/L4-HNE可通过caspase-3的激活引起支气管上皮细胞的凋亡。

Objective To explore the role of 4-hydroxynonenal （4-HNE） in bronchial epithelial cells（16-HBE） apoptosis. Methods 16-HBE cells were divided into four groups： control ,10 μmol/L, 30μmol/L,50 μmol/L 4-HNE stimulation groups . 16-HBE were stimulated by 10 μmol/L,30 μmol/L,50 μmol/L 4-HNE for 4 hours , and the apoptosis of cells were observed . The phosphorylation of JNK and caspase-3 were determined by Western blot. Results Cell apoptosis was obviously found in 30,50 μmol/L 4-HNE stimulating groups by Giemsa staining. The numbers of apoptosis in control , 10 μmol/L, 30 μmol/L, 50 μmol/L 4-HNE stimulation groups were 1.94 ± 1.03,2.03 ± 1.04,43.36 ± 1.3, and 65.92 ± 3.45 , respectively. There were significant differences of 30 and 50 μmol/L 4-HNE to control and 10 μmol/L 4-HNE groups （ P 〈 0.01 ）. The expression of phosph-JNK in four groups was not different , but the csapase-3 expression was significantly higher in 30,50 μmol/L 4-HNE groups compared to the control , 10 μmol/L 4-HNE groups. Conclusion 4-HNE induces apoptosis of bronchial epithelial cells, which may be related to activation of caspase-3.