家兔新基因Gnas的鉴定及其在高脂血症中的功能

Study of Function and Identification of Gnas,a Novel Gene Involved inRabbits with Hyperlipidemia

目的探讨家兔新基因Gnas在高脂血症中的功能。方法用高脂饲料复制家兔高胆固醇血症模型,分别用胆固醇、25羟胆固醇、低密度脂蛋白刺激HepG2细胞,采用荧光半定量逆转录聚合酶链反应技术检测GenBank中登录号为AY248719的家兔新基因表达情况。结果高胆固醇兔动物模型复制成功,该组血浆和肝脏的总胆固醇及肝脏甘油三酯水平增加均超过10倍,血浆甘油三酯也显著增高。高脂组Gnas基因表达水平明显高于对照组。胆固醇、25羟胆固醇刺激HepG2细胞后Gnas表达无明显改变,400 mg/L低密度脂蛋白刺激HepG2细胞后Gnas表达增强2.15倍。结论对家兔新基因Gnas的研究有助于进一步揭示高脂血症的分子机理。

Aim To study the role of a novel rabbits gene named Gnas during the hyperlipidemia hyperlipidemia, rabbits were obtained after feeding atherogenic diet for 150 d. The differentially expressed gene was identified by fluorescent semi-quantitative PCR. The different nucleic acid expression level of Gnas in HepG2 cell line induced by cholesterol, 25-hydryoxycholesterol, and human low density lipoprotein was analyzed by semi-quantitive RT-PCR. Results The hyperlipidemia rabbits model has been successfully prepared. The total cholesterol levels in plasma and liver, triglyceride level in liver of hypereholesterolemia rabbits increased more than 10 folds, triglyceride level in plasma also significantly increased compared with those in control group, mRNA expression enhanced in the liver of hyperlipidemia rabbits. 400ug/ml LDL induced Gnas expression increased 2.15 folds in HepG2 cell, though the same induced effects were not observed in cholesterol or 25-hydryoxycholesterol. Conclusions These results will contribute to reveal the molecular mechanism of the hyperlipidemia.