系统性红斑狼疮患者骨髓间充质干细胞细胞因子分泌及其与病情活动的相关性研究

Correlation of cytokine expression in bone marrow-derived mesenchymal stem cells with activity of systemic lupus erythematosus

目的探讨系统性红斑狼疮(SLE)患者骨髓间充质干细胞(MSCs)细胞因子分泌及其与疾病活动的相关性。方法采用密度梯度离心和贴壁分离法对11例SLE患者和6例正常人骨髓MSCs进行分离培养,流式细胞术鉴定MSCs。取P2代细胞,半定量RT-PCR检测SLE患者骨髓MSCs白细胞介素-6(IL-6),IL-7,IL-11,巨噬细胞集落刺激因子(M-CSF)和干细胞因子(SCF)的表达。结果SLE患者MSCs均表达CD29、CD44和CD105,不表达CD14、CD34、CD45和HLA-DR。两组P2代MSCs均表达IL-6、IL-7、IL-11、M-CSF和SCF。SLE患者组MSCsIL-6、IL-7表达降低(P<0.01),IL-7的表达和SLE的活动评分(SLEDAI)呈负相关(r=-0.891,P<0.05)。结论SLE患者MSCs细胞因子分泌异常,可能与SLE血液系统损害及病情活动相关。

Objective To explore the cytokine expression of bone marrow-derived mesenchymal stem cells （MSC） and its relationship with activity of systemic lupus erythematosus （SLE）. Methods MSC were isolated from bone marrow of both 11 SLE patients and 6 healthy controls by density centrifugation and adhesive culture in vitro. The surface markers were detected by flow cytometry. Semi-quantitative RT-PCR was used to detect the mRNA expressions of IL-6, IL-7, IL-11, stem cell factor （SCF） and macrophage colony ~timulating factor （M-CSF）. Results Both groups were positive for CD29, CD44 and CD105, and negative for CD14, CD34, CD45 and HLA-DR and expressed IL-6, IL7, IL-11, M-CSF and SCF . While IL-6 and IL-7 were down-regulated in MSCs from SLE patients （P 〈0.01 ）. IL-7 expression was negatively correlated with the SLE disease activity index （ SLEDAI ） in SLE patients （ r = - 0. 891, P 〈 0. 05 ）. Conclusions MSC in patient with SLE have abnormality in cytokines expression compared with those in normal control. MSC in patient with SLE may participate in hematological involvement and relate to lupus activity.