不同细胞 DNA 氧化损伤及自身修复能力的分析

THE DIFFERENCES OF DNA DAMAGE AND REPAIR BETWEEN SEVERAL TYPES OF CELLS

本文利用先进的“彗星”电泳技术分析了不同种类细胞ＤＮＡ氧化损伤及自身修复能力。结果显示Ｈｅｌａ、ＧＭ１８９９、ＨｅｐＧ２、分化的成纤维细胞对Ｈ２Ｏ２引起的损伤具有高度敏感性。当Ｈ２Ｏ２剂量达到１００μＭ时，四种细胞ＤＮＡ损伤率达到９８—９９％。然而淋巴细胞在１００μＭＨ２Ｏ２剂量处理后的ＤＮＡ损伤率仅有４２．１％，显示出对氧化损伤具有较高的抵抗力。在分析ＤＮＡ修复能力时发现，ＤＮＡ损伤率达到９８％的Ｈｅｌａ细胞在经过１ｈ的培养后其自身修复率为９０．１％，而淋巴细胞修复率仅有５１．１％，这说明Ｈｅｌａ细胞比淋巴细胞有较强的、快速的ＤＮＡ修复能力。

The analysis of DNA damage and repair was made with 'comet assay'.The results showed that Hela,GM1899,HepG2 and fibroblast cells were more sensitive to H 2O 2 than lymphocytes from the peripheral blood.When the dosage of H 2O 2 was 100μM,the rates of DNA damage in former types of cells were 98￣99%;however,that of lymphocytes only was 42.1%.With regard to DNA repair,another result showed the repair rate of Hela cells with 98% DNA damage reached 90.1% after 1 hour incubation,and there was 51.1% in lymphocytes.It is suggested that there is a more repair after DNA damage in Hela cells than lymphocytes.