新型二异羟肟酸配合物催化PNPP水解的动力学研究

Kinetic study on the hydrolysis of PNPP catalyzed by dihydroxamic acids open chain crown ether complexes

以3种含中心功能基(-OCH3)的二异羟肟酸开链冠醚过渡金属铜(Ⅱ)、锌(Ⅱ)和钴(Ⅱ)配合物作为水解酶模型,催化羧酸酯(PNPP)水解;研究了水解反应的动力学和机理,提出了水解反应的动力学模型。结果表明:在室温25℃条件下,这类配合物催化PNPP水解速率随着缓冲溶液pH值的增大而提高,表现出高的催化活性。

Three dihydroxamic acids open chain crown ether containing central functional group （ - OCH3 ） transition- metal complexes were used as hydrolytic enzyme -mimic model to catalyze carboxylic acid esters （PNPP） hydrolysis. The kinetics and the mechanism of PNPP hydrolysis catalyzed by these complexes were investigated. The mathematical kinetics model of PNPP cleavage catalyzed by these complexes was also proposed. The results showed that the rate of the PNPP hydrolysis catalyzed by the complexes is remarkably accelerated with the increasing of pH of the buffer solution and these complexes exhibited high activity in the PNPP catalytic hydrolysis.