兔VX2肝癌模型的肝动脉插管技术改良及DSA表现

The improvement of catheterization technique in rabbit VX2 hepatoma model and its manifestation in DSA

目的:探讨对兔VX2肝癌模型肝动脉插管技术的改良,并研究其DSA造影表现。方法:制作36只兔VX2肝癌模型。实验组(26只)采取改良显微外科手术直接行肝动脉插管法行介入治疗,对照组(10只)采用3F微导管经股动脉-肝动脉插管法作对比研究。术前及术后用DSA造影方法进行兔VX2肝癌的影像学观察。结果:实验组的插管成功率为88%(23/26),对照组的插管成功率为40%(4/10)。27只插管成功模型DSA造影示瘤周血管增粗紊乱、大量细小的异常血管,其中24只肿瘤呈均一的或结节样染色。结论:采用显微外科手术改良的肝动脉插管法对兔VX2肝癌模型行介入治疗成功率较高,且可减少实验人员接受的X线辐射,DSA造影可较好评价肿瘤血供情况,是一种较好的实验方法。

To explore the improvement of catheterization technique in rabbit VX2 hepatoma model and its manifestation in DSA. Methods A VX2 hepatoma model was constructed in 36 rabbits. 26 of the rabbits subsequently underwent improved microsurgical catheterization of hepatic artery for intervention therapy （experimental group）, while the remaining 10 were catheterized with 3F micro-catheter by Seldinger technique （control group）. VX2 hepatoma lesions were detected pre- and post-operatively using DSA. Results The success rate of catheterization was 88% （23/26） in the experimental group and 40% （4/10） in the control group. Hepatic artery angiography showed that the surrounding vessels of the tumor were distended and disrupted. The aberrant vessels and malignant lesions were homogeneous or nodous in stain. Conclusions The modified microsurgical catheterization of hepatic artery has a higher success rate in the rabbit VX2 hepatoma model and can decrease researchers＇ exposure to X-ray. This new procedure is promising and DSA is ideal in assessing the blood supply to the tumor.