摘要
目的为了解HBV慢性感染免疫耐受期患者肝内NK细胞及Kupffer细胞的变化特点,探讨肝内NK细胞及Kupffer细胞与HBV慢性感染免疫耐受的关系。方法采用免疫组织化学方法检测16例HBV感染免疫耐受期患者肝组织内NK细胞及Kupffer细胞在肝内分布情况,以及HBsAg、HBcAg在肝细胞内表达状况,并与6例正常肝组织、17例免疫活动期患者进行比较。结果免疫耐受期患者肝内浸润Kupffer细胞明显多于正常肝组织(P<0.01),但明显少于免疫活动期患者(P<0.01);免疫耐受期患者肝内NK细胞数与免疫活动期及正常肝组织比较均差异无显著性(P>0.05);免疫耐受期患者肝细胞内HBcAg阳性表达明显高于免疫活动期患者(P<0.01)。结论肝组织内Kupffer细胞在慢性乙型肝炎的肝组织炎症损伤及肝内HBV清除中起重要作用,而慢性HBV感染者肝组织内NK细胞的作用很有限。
[Objective] To investigate the changing characteristics of intrahepatic NK ceils and Kupffer ceils among patients in immune tolerant phase. Meanwhile, to explore the relationship between intrahepatic different immune competent cells and the status of immune tolerance in chronic HBV infection. [Methods] 16 liver biopsies were selected from HBV infection patients in immune tolerant phase for detecting of NK ceils and Kupffer ceils in the liver biopsies of patients and determination of the characteristics of HBsAg and HBeAg expressed in hepatocytes with immunohistochemical staining. Six normal liver biopsies and 17 liver biopsies of patients in immuneactive phase were employed to serve as the control respectively. [Results] It was found that Kupffer ceils in the liver biopsies of patients in immune tolerant phase were significantly increased as compared with normal control （t =5.441, P 〈0.01） and significantly decreased as compared with the patients in immuneactive phase （t =5.062, P 〈0.01）. The number of NK ceils in the liver biopsies was almost the same among patients with the HBV infection at different phases and the control （P 〉0.05）. The rate of HBcAg expressing in hepatocytes of patients at immune tolerant phase was significantly higher than that of patients at immuneactive phase （t =8.76, P 〈0.01）. [Conclusions] Kupffer ceils may play important roles in clearance of H/3V virus and in liver inflammation and injury in chronic HBV infection. And NK ceils plays an unimportant roles.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2007年第11期1330-1333,共4页
China Journal of Modern Medicine
