麒麟菜硫酸酯多糖体外抗病毒作用的研究

Antiviral activities of polysaccharides from Eucheuma gelatinae and Eucheuma striatum in vitro

目的采用体外细胞培养法,研究琼枝和异枝麒麟菜硫酸酯多糖对HSV-1和CVB3的抗病毒活性,并初步探讨其作用机制。方法采用MTT比色法观察琼枝和异枝麒麟菜硫酸酯多糖对Vero细胞的细胞毒性,用细胞病变(CPE)抑制法观察其对由HSV-1和CVB3引起CPE的抑制作用;采用分组实验初步探讨了多糖样品的抗病毒作用机制。结果和结论琼枝和异枝麒麟菜硫酸酯多糖能明显抑制由上述两种病毒引起的CPE,对Vero细胞的毒性很低,异枝粗多糖和异枝多糖A2组分(ESPA和ESPA2)对HSV-1的抗病毒效果均优于阿昔洛韦(ACV),本实验采用的全部多糖样品对CVB3的抗病毒作用也都优于病毒唑,而异枝麒麟菜硫酸酯多糖抗CVB3效果更为显著。从分组实验结果可初步推知,麒麟菜硫酸酯多糖样品不仅具有直接杀灭病毒作用,而且还可能通过进入细胞内部或吸附在细胞表面,发挥其抑制或杀伤病毒的作用。

Objective The antiviral effects of polysaccharides from Eucheuma gelatinae and Eucheuma striatum on herpes simplex virus type 1 （HSV-1） and coxsackie virus （CVB3） in vitro were assayed and their antiviral mechanism was elucidated. Methods The antiviral effects of samples were evaluated with cell cytotoxicity by MTT and cytopathic effect（CPE） methods. Antiviral mechanism of two extracts was investigated by four different ways. Results and Conclusion Polysaccharides from Eucheuma gelatinae and Eucheuma striatum had an obviously HSV-1 and CVB3 inhibitory effect. Antiviral activities of two extracts （ESPA and ESPA2） were better than acyclovir （ACV）. Antiviral activities of all extracts were better than ribavirin injection. Polysaccharides from Eucheuma striatum had remarkable antiCVB3 effects. The experiment also showed that Eucheuma polysaccharides not only kill the above viruses directly but also restrain them via getting into cells or absorbing on cells.